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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in several tumors, which leads to chromosome instability, and so may well contribute to tumorigenesis. This protein was observed to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial purpose in advertising cell-cycle development in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are already explained. Two further splice variants were reported but in-depth nucleotide sequence information and facts isn't yet readily available.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide usually incorporates the epitope identified via the antibody.
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This guidebook outlines even further achievable optimization of mobile and immunoassay parameters to be certain the very best benefits are...
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This information outlines more possible optimization of cellular and immunoassay parameters to be sure the very best success are...
Antibodies bound to the blocking peptide not bind into the epitope on the goal protein. By evaluating the staining within the blocked antibody versus the antibody by yourself, you can see which staining is unique.
Goal Information and facts Cyclin E belongs for the highly conserved cyclin relatives, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinctive expression and degradation designs which contribute to your temporal coordination of each mitotic party. Cyclin E kinds a complex with and features as being a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S changeover. This protein accumulates on the G1-S section boundary which is thr77 degraded as cells progress as a result of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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